Ubiquitin Antikörper

Produktnummer ABIN336952

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Ubiquitin in IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Rind (Kuh) und Pferd.

Kurzübersicht für Ubiquitin Antikörper (ABIN336952)

Target: Ubiquitin (Ub)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ubiquitin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Ubiquitin Antikörper

Spezifität: Molecular Weight: 8.5 kDa.

Immunogen: Purified human Ubiquitin

Anwendungsinformationen

Applikationshinweise: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:100 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. FFPE tissue sections require antigen retriever, boiling tissue for 15-20 minutes in 10 mM citrate , pH 6.0, followed by cooling at RT for 15-20 minutes The optimum dilution should be determined by the individual lab. Positive Control: Human brain from Alzheimer’s disease. Cellular Localization: Nuclear, Cytoplasmic, and cell membrane.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The antibody is supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu Ubiquitin

Target: Ubiquitin (Ub)

Andere Bezeichnung: Ubiquintin

Hintergrund: Ubiquitin is a 76 amino acids 8.5 kDa, a highly conserved small regulatory protein that is ubiquitously expressed in eukaryotes and plays a central role in protein degradation. The Ubiquitin/proteosome pathways are main, non-lysomal route for intracellular protein degradation. The protein to be degraded is conjugated to Ubiquitin, it is selectively degraded by 26S complex multicatalytic cytosolic and nuclear protease termed proteosome. This protein also plays important role in cellular processes e.g. Cell cycle progression, gene transcription, antigen processing and signal transduction. In Immunohistochemistry (IHC) this antibody can detect abnormal inclusion bodies, in neurofibrillary tangles in Alzheimer’s disease, lewy bodies in Parkinson’s disease, pick bodies in Pick’s disease, inclusions in motor neuron disease, Mallory’s Haylin in alcoholic liver disease and Rosenthal fibers in astrocytes. This antibody stains senile plaques and neurons.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway