Keratin 10/13 Antikörper

Produktnummer ABIN336868

Dieser Maus Monoklonal Antikörper detektiert spezifisch Keratin 10/13 in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Keratin 10/13 Antikörper (ABIN336868)

Target: Keratin 10/13

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: CK208

Produktdetails

Spezifität: Molecular Weight: CK 10 is 56.9 kDa and CK 13 is 53 kDa.

Immunogen: Cytokeratin extraction from human ectocervical epithelium

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (IHC), Immunocytochemistry (ICC) (frozen tissue sections ONLY or cell smears) For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. The optimum dilution should be determined by the individual lab. Positive Control: Human tonsil or skin. Cellular Localization: Cytoplasmic.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The concentrated antibodies are in supplied in PBS with 1% BSA, 0.05% azide, pH 7.4.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Antigendetails

Target: Keratin 10/13

Andere Bezeichnung: Cytokeratin 10+13

Hintergrund: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20. This antibody reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. With frozen sections, this antibody serves as a differentiation related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Recently Silveira et. al. (3) have reported that in tongue squamous cell carcinoma the expression of CK 10 was significantly correlated with the presence of metasis.