Keratin 10/13 Antikörper

Produktnummer ABIN7865677

Der Maus Monoklonal anti-Keratin 10/13 Antikörper (Klon EBS-IF-003) (ABIN7865677) detektiert spezifisch Keratin 10/13 in WB, FACS, IF, IHC und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Cat und Hund.

Kurzübersicht für Keratin 10/13 Antikörper (ABIN7865677)

Target: Keratin 10/13

Reaktivität: Human, Cat, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Klon: EBS-IF-003

Produktdetails

Verwendungszweck: anti-Keratin-13+10 mouse monoclonal, EBS-IF-003, purified

Aufreinigung: affinity chromatography

Immunogen: cytoskeleton preparation from HeLa cells

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonon vor 15 min, at 4 °C

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Antigendetails

Target: Keratin 10/13

Andere Bezeichnung: Keratin-13+10

Hintergrund: EBS-IF-003 reacts with 53 kDa (CK13) and 56.6 kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis. Positive control: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.