S100A9 Antikörper (Internal Region)

Produktnummer ABIN335161

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch S100A9 in WB, IHC, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für S100A9 Antikörper (Internal Region) (ABIN335161)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser S100A9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-S100A9 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: S100A9

Sequenz: DTNADKQLSF EEF

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DTNADKQLSFEEF

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Lung. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 13-14 kDa band observed in Human Bone Marrow lysates and approx 14-15 kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2 kDa according to NP_002956.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour a

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended conc

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Andere Bezeichnung: S100A9

Hintergrund: S100A9, S100 calcium binding protein A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B

Gen-ID: 6280

NCBI Accession: NP_002956

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine