ZEB1 Antikörper (Internal Region)

Produktnummer ABIN335118

Der Ziege Polyklonal anti-ZEB1 Antikörper (ABIN335118) detektiert spezifisch ZEB1 in WB, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für ZEB1 Antikörper (Internal Region) (ABIN335118)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ZEB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ZEB1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: AREB6 / ZEB1

Sequenz: RPQIRQKIEN KPLQE

Spezifität: This antibody is expected to recognize both reported isoforms (NP_001121600.1, NP_110378.3).

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RPQIRQKIENKPLQE, from the internal region of the protein sequence according to NP_001121600.1, NP_110378.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx.100 kDa band observed in Mouse Heart lysates (calculated MW of 122 kDa according to Human NP_110378.3 and Mouse NP_035676.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Andere Bezeichnung: ZEB1

Hintergrund: ZEB1, AREB6, zinc finger E-box binding homeobox 1, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A, OTTHUMP00000019405, transcription factor 8 (represses interleukin 2 expression), zinc finger homeodomain enhancer-binding prote

Gen-ID: 6935, 21417, 25705

NCBI Accession: NP_001121600, NP_110378

Pathways: Regulation of Muscle Cell Differentiation