HNF1A Antikörper (Internal Region)

Produktnummer ABIN334504

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch HNF1A in ELISA, FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für HNF1A Antikörper (Internal Region) (ABIN334504)

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HNF1A Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-HNF1A Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TCF1 / HNF1

Sequenz: DELPTKKGRR NRFK

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DELPTKKGRRNRFK, from the internal region of the protein sequence according to NP_000536.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: A customer reported a weak band at approx. 75 kDa on lysate of cell line Caco-2, at a concentration of 3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HepG2 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Andere Bezeichnung: HNF1A

Hintergrund: TCF1, transcription factor 1, hepatic LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor , HNF1, HNF1A, LFB1, MODY3 , Interferon production regulator factor (HNF1), albumin proximal factor, hepatic nuclear factor 1, maturity onset diabetes of t

Gen-ID: 6927, 21405, 24817

NCBI Accession: NP_000536

Pathways: Hormone Transport, Carbohydrate Homeostasis