MGLL Antikörper (Internal Region)

Produktnummer ABIN334436

Der Ziege Polyklonal Anti-MGLL-Antikörper wurde für WB, IHC, ELISA, FACS und IF validiert. Er ist geeignet, MGLL in Proben von Human und Maus zu detektieren.

Kurzübersicht für MGLL Antikörper (Internal Region) (ABIN334436)

Target: MGLL (Monoglyceride Lipase (MGLL))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MGLL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-MGLL Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Monoglyceride Lipase

Sequenz: QDLPHLVNAD GQY

Spezifität: This antibody is expected to recognize both reported isoforms (NP_009214.1, NP_001003794.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1, NP_001003794.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3 kDa according to NP_001003794.1). Recommended concentration: 0.3-1 μg/mL. Pri

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu MGLL

Target: MGLL (Monoglyceride Lipase (MGLL))

Andere Bezeichnung: MGLL

Hintergrund: Monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like

Gen-ID: 11343, 23945, 29254

NCBI Accession: NP_009214, NP_001003794

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism