MBD2 Antikörper (Internal Region)
Kurzübersicht für MBD2 Antikörper (Internal Region) (ABIN334432)
Target
Alle MBD2 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
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Bindungsspezifität
- Internal Region
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Verwendungszweck
- MBD2 (isoform 1)
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Sequenz
- RNDPLNQNKG KPDLN
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Spezifität
- This antibody is expected to resognize isoform 1 (NP_003918.1) only.
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Kreuzreaktivität
- Hund, Human, Maus, Ratte
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Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.
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Isotyp
- IgG
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Applikationshinweise
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Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio
Peptide ELISA: antibody detection limit dilution 1:64000.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).
: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." in: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).
: "
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A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).
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- MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))
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Andere Bezeichnung
- MBD2
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Hintergrund
- MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41
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Gen-ID
- 8932, 17191
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NCBI Accession
- NP_003918
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Pathways
- Chromatin Binding
Target
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