MBD2 Antikörper (Internal Region)

Produktnummer ABIN334432

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch MBD2 in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für MBD2 Antikörper (Internal Region) (ABIN334432)

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MBD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-MBD2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: MBD2 (isoform 1)

Sequenz: RNDPLNQNKG KPDLN

Spezifität: This antibody is expected to resognize isoform 1 (NP_003918.1) only.

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio

Peptide ELISA: antibody detection limit dilution 1:64000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-MBD2 Antikörper (ABIN334432)

Bartels, Spruijt, Brinkman, Jansen, Vermeulen, Stunnenberg: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).

Spruijt, Bartels, Brinkman, Tjeertes, Poser, Stunnenberg, Vermeulen: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." in: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).

Details zu MBD2

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Andere Bezeichnung: MBD2

Hintergrund: MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41

Gen-ID: 8932, 17191

NCBI Accession: NP_003918

Pathways: Chromatin Binding