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MBD2 Antikörper (Internal Region)

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch MBD2 in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.
Produktnummer ABIN334432

Kurzübersicht für MBD2 Antikörper (Internal Region) (ABIN334432)

Target

Alle MBD2 Antikörper anzeigen
MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Reaktivität

  • 48
  • 24
  • 7
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Wirt

  • 46
  • 2
Ziege

Klonalität

  • 42
  • 6
Polyklonal

Konjugat

  • 31
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MBD2 Antikörper ist unkonjugiert

Applikation

  • 41
  • 17
  • 9
  • 7
  • 6
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse

Verified
  • Bindungsspezifität

    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Verwendungszweck

    MBD2 (isoform 1)

    Sequenz

    RNDPLNQNKG KPDLN

    Spezifität

    This antibody is expected to resognize isoform 1 (NP_003918.1) only.

    Kreuzreaktivität

    Hund, Human, Maus, Ratte

    Aufreinigung

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Minimize freezing and thawing.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Bartels, Spruijt, Brinkman, Jansen, Vermeulen, Stunnenberg: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).

    Spruijt, Bartels, Brinkman, Tjeertes, Poser, Stunnenberg, Vermeulen: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." in: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).

  • Target

    MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

    Andere Bezeichnung

    MBD2

    Hintergrund

    MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41

    Gen-ID

    8932, 17191

    NCBI Accession

    NP_003918

    Pathways

    Chromatin Binding
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