ITCH Antikörper (Internal Region)

Produktnummer ABIN334426

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ITCH in ELISA, WB, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ITCH Antikörper (Internal Region) (ABIN334426)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ITCH Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-ITCH Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ITCH / AIF4

Sequenz: EIKSHDLKPN GGN

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 90 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4 kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Andere Bezeichnung: ITCH

Hintergrund: ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiqui

Gen-ID: 83737, 16396, 311567

NCBI Accession: NP_113671, NP_001244066, NP_001244067

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events