HEXIM1 Antikörper (C-Term)

Produktnummer ABIN334418

Der Ziege Polyklonal Anti-HEXIM1-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, HEXIM1 in Proben von Human und Maus zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für HEXIM1 Antikörper (C-Term) (ABIN334418)

Target: HEXIM1 (Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HEXIM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-HEXIM1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: HEXIM1

Sequenz: HRQQERAPLS KFGD

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HRQQERAPLSKFGD, from the C Terminus of the protein sequence according to NP_006451.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 40 kDa band observed in Human and Mouse Heart lysates (calculated MW of 40.6 kDa according to Human NP_006451.1 and 40.2 kDa according to Mouse NP_620092.1. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room tem

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nucleus of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-HEXIM1 Antikörper (ABIN334418)

Bugai, Quaresma, Friedel, Lenasi, Düster, Sibley, Fujinaga, Kukanja, Hennig, Blasius, Geyer, Ule, Dölken, Barborič: "P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress." in: Molecular cell, Vol. 74, Issue 2, pp. 254-267.e10, (2019) (PubMed).

Faust, Li, Bacon, Jang, Weiss, Jayaraman, Newton, Krogan, DOrso, Frankel: "The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation." in: eLife, Vol. 7, (2019) (PubMed).

Details zu HEXIM1

Target: HEXIM1 (Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1))

Andere Bezeichnung: HEXIM1

Hintergrund: HEXIM1, hexamethylene bis-acetamide inducible 1, HGNC:24953, CLP1, HIS1, MAQ1 , HMBA-inducible, hexamethylene bisacetamide-inducible protein, hexamethylene-bis-acetamide-inducible transcript 1, hexamthylene bis-acetamide inducible 1, menage a quatre 1, ED

Gen-ID: 10614, 192231

NCBI Accession: NP_006451