GADD45G Antikörper (Internal Region)

Produktnummer ABIN334406

Der Ziege Polyklonal Anti-GADD45G Antikörper wurde für ELISA, IF und FACS validiert. Er ist geeignet, GADD45G in Proben von Human zu detektieren.

Kurzübersicht für GADD45G Antikörper (Internal Region) (ABIN334406)

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GADD45G Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-GADD45G Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: GADD45gamma (aa 18 to 28)

Sequenz: RMQGAGKALH E

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RMQGAGKALHE, from the internal region of the protein sequence according to NP_006696.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary testing showed a band at approx 23 kDa in lysate of cell line A549 and in Human Prostate and Testes lysate at a concentration of 0.5-1 μg/mL (calculated Mwt.of 17.1 kDa according to NP_006696.1). This molecular weight is observed b

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10u

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu GADD45G

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Andere Bezeichnung: GADD45G

Hintergrund: Growth arrest and DNA-damage-inducible, gamma, CR6, DDIT2, GADD45gamma, GRP17, GADD45-gamma, gadd-related protein, 17 kD, growth arrest and DNA-damage-inducible gamma

Gen-ID: 10912, 23882, 291005

NCBI Accession: NP_006696

Pathways: Zellzyklus