CXCR3 Antikörper (C-Term)

Produktnummer ABIN334381

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CXCR3 in FACS, ELISA, IHC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CXCR3 Antikörper (C-Term) (ABIN334381)

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CXCR3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-CXCR3 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: CXCR3 / GPR9

Sequenz: RRDSSWSETS EA

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RRDSSWSETSEA, from the C Terminus of the protein sequence according to NP_001495.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

Western Blot: Preliminary experiments showed a band at approx 55-60 kDa in Human Tonsil and Mouse Spleen lysates after 1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at r

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu CXCR3

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Andere Bezeichnung: CXCR3

Hintergrund: CXCR3, chemokine (C-X-C motif) receptor 3, HGNC:4540, CD183, CKR-L2, CMKAR3, GPR9, IP10, IP10-R, Mig-R, MigR, G protein-coupled receptor 9, IP10 receptor, Mig receptor, chemokine (C-X-C) receptor 3

Gen-ID: 2833, 12766, 84475

NCBI Accession: NP_001495, NP_001136269