EEF2 Antikörper (pThr56)

Produktnummer ABIN334266

Dieser Anti-EEF2 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von EEF2 in WB, IHC, ELISA und IHC (p). Geeignet für Human, Maus, Ratte, Huhn, Affe und Hamster.

Kurzübersicht für EEF2 Antikörper (pThr56) (ABIN334266)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EEF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EEF2 Antikörper

Bindungsspezifität: pThr56

Spezifität: Detects endogenous levels of eEF2 only when phosphorylated at threonine 56. It does not recognize eEF2 phosphorylated at other sites.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr56 of human eEF2.

Type of Immunogen: Synthetic phospho-peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (1:100), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4uC with gentle shaking, overnight.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Andere Bezeichnung: EEF2 / Elongation Factor 2

Hintergrund: Name/Gene ID: EEF2

Synonyms: EEF2, EEF-2, EF-2, Elongation factor 2, Polypeptidyl-tRNA translocase, EF2

Gen-ID: 1938

UniProt: P13639

Pathways: AMPK Signaling