NUMB Antikörper

Produktnummer ABIN324854

Der Schaf Polyklonal Anti-NUMB-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, NUMB in Proben von Human zu detektieren.

Kurzübersicht für NUMB Antikörper (ABIN324854)

Target: NUMB (Numb Homolog (NUMB))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser NUMB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-NUMB Antikörper

Spezifität: Recognizes human Numb. Species sequence homology: mouse: 88 % and canine: 90 %.

Aufreinigung: Immunoaffinity purified

Immunogen: E. coli-derived recombinant human rhNumb.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in Western blot, Immunocytochemistry, and Direct ELISA. Western blot: 1.0 μg/mL. Immunocytochemistry: 10 μg/mL Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. Follow by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.1 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Details zu NUMB

Target: NUMB (Numb Homolog (NUMB))

Andere Bezeichnung: NUMB

Hintergrund: Name/Gene ID: NUMB

Synonyms: NUMB, C14_5527, Numb (Drosophila) homolog, S171, Protein numb homolog, C14orf41, H-Numb, Numb homolog (Drosophila), Protein S171

Gen-ID: 8650

UniProt: P49757

Pathways: Cell-Cell Junction Organization