Notch1 Antikörper (AA 2428-2556)

Produktnummer ABIN324719

Der Maus Monoklonal Anti-Notch1-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, Notch1 in Proben von Human zu detektieren.

Kurzübersicht für Notch1 Antikörper (AA 2428-2556) (ABIN324719)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Notch1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-Notch1 Antikörper

Bindungsspezifität: AA 2428-2556

Spezifität: Recognizes human Notch-1 IC.

Aufreinigung: Protein A/G purified

Immunogen: E. coli-derived rhNotch-1 IC. (aa 2428 - 2556).

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Direct ELISA: 0.5-1.0 μg/mL detection limit for rhNotch-1 IC is ~3 ng/well. Western Blot: 1-2 μg/mL detection limit for rhNotch-1 IC is ~25 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL. For SAOS2 osteosarcoma cells, cells were fixed with PBS containing 4 % paraformaldehyde for 20 min at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100 and 1 % BSA for 45 min at RT. After blocking, cells were incubated with primary antibody overnight at 4?C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized, PBS, 5 % trehalose

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Andere Bezeichnung: NOTCH1

Hintergrund: Name/Gene ID: NOTCH1

Synonyms: NOTCH1, Notch 1, TAN1

Gen-ID: 4851

UniProt: P46531

Pathways: Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development