HMGB1 Antikörper (AA 126-175)

Produktnummer ABIN324443

Der Kaninchen Polyklonal Anti-HMGB1-Antikörper wurde für WB validiert. Er ist geeignet, HMGB1 in Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Schwein, Kaninchen, Affe, Meerschweinchen, Pferd, Huhn, Hamster und Fledermaus zu detektieren.

Kurzübersicht für HMGB1 Antikörper (AA 126-175) (ABIN324443)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein, Kaninchen, Affe, Meerschweinchen, Pferd, Huhn, Hamster, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-HMGB1 Antikörper

Bindungsspezifität: AA 126-175

Spezifität: Human HMGB1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Xenopus, Zebrafish (92%).

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide located between aa126-175 of human HMGB1 (P09429, NP_002119). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Smelt (100%), Xenopus, Frog, Zebrafish (92%), Trout, Salmon, Sablefish (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: HMG1 / HMGB1

Hintergrund: Name/Gene ID: HMGB1

Synonyms: HMGB1, Amphoterin, High mobility group protein B1, High mobility group box 1, High mobility group protein 1, HMG1, HMG3, SBP-1, High-mobility group box 1, HMG-1

Gen-ID: 3146

NCBI Accession: NP_002119

UniProt: P09429

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome