FAS Antikörper (Extracellular Domain) (PE)

Produktnummer ABIN323507

Produktdetails anti-FAS Antikörper (PE)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Bindungsspezifität: Extracellular Domain

Reaktivität: Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FAS Antikörper ist konjugiert mit PE

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Spezifität: Recognizes feline Fas extracellular domain. Species sequence homology: human 68 % , mouse 65 %, porcine 53 %, and rat 58 %. Does not cross-react with rhFas, rmFas, or rrFas.

Aufreinigung: Protein G purified

Immunogen: NS0-derived recombinant feline rfeFas extracellular domain.

Type of Immunogen: Recombinant protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 μg/mL. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Cat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized powder from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Details zu FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Andere Bezeichnung: CD95 / FAS (FAS Produkte)

Synonyme: ALPS1A antikoerper, APO-1 antikoerper, APT1 antikoerper, CD95 antikoerper, FAS1 antikoerper, FASTM antikoerper, TNFRSF6 antikoerper, AI196731 antikoerper, APO1 antikoerper, TNFR6 antikoerper, Tnfrsf6 antikoerper, lpr antikoerper, Fas cell surface death receptor antikoerper, Fas (TNF receptor superfamily member 6) antikoerper, FAS antikoerper, Fas antikoerper, fas antikoerper

Hintergrund: Name/Gene ID: FAS
Family: TNF Receptor

Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95

Gen-ID: 355

UniProt: P25445

Pathways: p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity