CD3D Antikörper (Chain delta) (FITC)

Produktnummer ABIN320297

Produktdetails anti-CD3D Antikörper (FITC)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Bindungsspezifität: Chain delta

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3D Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Spezifität: SM253F recognises rat CD3, a 25 kDa antigen which is found on rat T cells. SM253F does not react with rat B cells.

Immunogen: F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.

Klon: 1F4

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Use 10 μL of Neat antibody to label 10^6 cells in 100 μL. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T- cells in the presence of PMA (2). We recommend antibody ABIN120367 for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) -20 °C for longer.

Details zu CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Andere Bezeichnung: CD3 delta Chain (CD3D Produkte)

Synonyme: CD3-DELTA antikoerper, T3D antikoerper, CD3 antikoerper, T3d antikoerper, CD3d molecule antikoerper, CD3 antigen, delta polypeptide antikoerper, CD3D antikoerper, Cd3d antikoerper

Hintergrund: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: CD3D, T-cell surface glycoprotein CD3 delta chain, T3D

Gen-ID: 25710

NCBI Accession: NP_037301

UniProt: P19377

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events