MDM4-binding Protein Antikörper (pSer367)

Produktnummer ABIN3201009

Dieser Maus Monoklonal Antikörper detektiert spezifisch MDM4-binding Protein in WB, ELISA und IP. Es zeigt Reaktivität gegenüber Proben von Human und Maus. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für MDM4-binding Protein Antikörper (pSer367) (ABIN3201009)

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MDM4-binding Protein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: #15

Produktdetails anti-MDM4-binding Protein Antikörper

Bindungsspezifität: pSer367

Produktmerkmale: Product: Mouse monoclonal antibody (clone #15) specific to the MdmX protein phosphorylated at Ser367, Human and mouse MdmX proteins phosphorylated at Ser367

Aufreinigung: Purified

Immunogen: A synthetic peptide corresponding to a sequence of human Mdmx protein surrounding pSer367

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: ~1 g/mL
2. Immunoprecipitation
3. ELISA
4. Indirect immuno-staining

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: -20 C (For long term storage: -70 C)

Referenzen für anti-MDM4-binding Protein Antikörper (ABIN3201009)

Chen, Gilkes, Pan, Lane, Chen: "ATM and Chk2-dependent phosphorylation of MDMX contribute to p53 activation after DNA damage." in: The EMBO journal, Vol. 24, Issue 19, pp. 3411-22, (2005) (PubMed).

Okamoto, Kashima, Pereg, Ishida, Yamazaki, Nota, Teunisse, Migliorini, Kitabayashi, Marine, Prives, Shiloh, Jochemsen, Taya: "DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9608-20, (2005) (PubMed).

Details zu MDM4-binding Protein

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Andere Bezeichnung: Mdmx/Hdmx

Hintergrund: MdmX (synonyms: Mdm4, HdmX) inhibits p53-and p73-dependent cell cycle arrest and apoptosis by binding to the transcription activation domains of these proteins. MdmX consists of 490 amino acids with the molecular weight of 54,864 and contains a RIING-finger domain and a nuclear transport signal. It is known that the protein migrates aberrantly in SDS-PAGE at the position of an 80- kDa protein. MdmX is phosphorylated at Ser367 by Chk2 kinase downstream of ATM in response to DNA damage, and as a result, it binds to14-3-3 and is transported into nucleus where it is degraded by Mdm2. This process activates the p53 functions.

UniProt: O15151

Pathways: Zellzyklus