Fibroblasts, Epithelial Cells Antikörper (PE)

Produktnummer ABIN319868

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-Fibroblasts, Epithelial Cells Antikörper wird verwendet zum Nachweis von Fibroblasts, Epithelial Cells in Proben von Human. Er wurde validiert für FACS.

Kurzübersicht für Fibroblasts, Epithelial Cells Antikörper (PE) (ABIN319868)

Target: Fibroblasts, Epithelial Cells

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS)

Klon: D7-FIB

Produktdetails

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Human foreskin Fibroblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.25 mL (SM1214RT) or 1 mL (SM1214R) distilled water.

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Referenzen

Raaijmakers, Widmer, Narechania, Eichhoff, Freiberger, Wenzina, Cheng, Mihic-Probst, Desalle, Dummer, Levesque: "Co-existence of BRAF and NRAS driver mutations in the same melanoma cells results in heterogeneity of targeted therapy resistance." in: Oncotarget, Vol. 7, Issue 47, pp. 77163-77174, (2018) (PubMed).

Nazareth, Broderick, Simpson-Abelson, Kelleher, Yokota, Bankert: "Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5552-62, (2007) (PubMed).

Antigendetails

Target: Fibroblasts, Epithelial Cells

Andere Bezeichnung: Fibroplasts / Epithelial Cells

Hintergrund: A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.Synonyms: Fibroblast Marker, Fibroblasten