Integrin beta 4 Antikörper

Produktnummer ABIN319862

Der Maus Monoklonal Anti-Integrin beta 4-Antikörper wurde für WB, FACS, IP und IHC (fro) validiert. Er ist geeignet, Integrin beta 4 in Proben von Human zu detektieren.

Kurzübersicht für Integrin beta 4 Antikörper (ABIN319862)

Target: Integrin beta 4 (ITGB4)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 450-9D

Produktdetails anti-Integrin beta 4 Antikörper

Spezifität: This antibody reacts with beta 4 Integrin (CD104). This antibody recognises an extracellular epitope on the CD104 Molecule.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G.

Immunogen: Purified alpha 6/beta 4 Integrin from A431 cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (1/25-1/100): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Western blot. Immunohistochemistry on Frozen Sections (1/500-1/1000). Does not work on Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Andere Bezeichnung: CD104 / Integrin beta-4

Hintergrund: Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA), also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities. CD104 is expressed on epithelial cells, Schwann cells and some tumor cell lines.Synonyms: GP150, ITGB4

Gen-ID: 3691

NCBI Accession: NP_000204

UniProt: P16144

Pathways: Integrin Complex