Isocitrate Dehydrogenase Antikörper

Produktnummer ABIN3197487

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Isocitrate Dehydrogenase in WB und IF. Es zeigt Reaktivität gegenüber Proben von Cabbage, Mais (Zea mays), Tomato, Soybean, Red pepper, Potato, Pea, Oryza sativa, Nicotiana benthamiana und Arabidopsis thaliana. Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für Isocitrate Dehydrogenase Antikörper (ABIN3197487)

Target: Isocitrate Dehydrogenase (IDH)

Reaktivität: Cabbage, Mais (Zea mays), Tomato, Soybean, Red pepper, Potato, Pea, Oryza sativa, Nicotiana benthamiana, Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Isocitrate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-Isocitrate Dehydrogenase Antikörper

Verwendungszweck: Anti-IDH | Isocitrate dehydrogenase (Cellular [compartment marker] of mitochondrial matrix)

Kreuzreaktivität (Details): Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Glycine max, Lycopersicum chilense, Nicotiana benthamiana, Oryza sativa, Solanum lycopersicum, Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays
Not reactive in: Chlamydomonas reinhardtii

Homologie: Brachypodium distachyon,Brassica napus,Capsella rubella,Citrus sinensis,Hordeum vulgare,Malus x domestica,Medicago truncatula,Nicotiana tabacum,Phaseolus vulgaris,Theobroma cacao,Triticum aestivum,Vitis vinifera,Zea mays

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial

Anwendungsinformationen

Applikationshinweise: 1:400 (IF), 1 : 5 000 (WB)

Kommentare:

cellular [compartment marker] of mitochondrial matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-Isocitrate Dehydrogenase Antikörper (ABIN3197487)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Lee, Lee, Yoo, Duncan, Oh, Lee, Lee, Whelan, Hwang: "Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs." in: The Plant cell, Vol. 24, Issue 12, pp. 5037-57, (2013) (PubMed).

Szal, Jastrz?bska, Kulka, Lea: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." in: Planta, Vol. 232, Issue 6, pp. 1371-82, (2010) (PubMed).

Details zu Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Hintergrund:

Background: Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.

Additional information: Peptide used to elicit this antibody is not conserved in NADPH dependent anzymes, partially conserved across eukaryotic Idh subunits, Some conservation across bacterial which contain the NAD-dependent form of Idh (as opposed to the NADP-dependent form)

Molekulargewicht: 39 | 45 kDa (Arabidopsis thaliana)

Gen-ID: 816218

UniProt: P93032