Isocitrate Dehydrogenase Antikörper

Produktnummer ABIN3197487

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Isocitrate Dehydrogenase in WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana, Pisum sativum, Potato, Tomato und Mais (Zea mays) und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für Isocitrate Dehydrogenase Antikörper (ABIN3197487)

Target: Isocitrate Dehydrogenase (IDH)

Reaktivität: Arabidopsis thaliana, Pisum sativum, Potato, Tomato, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Isocitrate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Isocitrate Dehydrogenase Antikörper

Kreuzreaktivität (Details): No cross-reactivity with: Chlamydomonas reinhardtii

Homologie: dicots including Brassica napus, Vitis vinifera, monocots including Oryza sativa, Zea mays

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 39 / 45 kDa (Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial, NAD dependent isocitrate dehydrogenase subunits including Arabidopsis thaliana IDH-I Q8LFC0, At4g35260 and IDH-II P93032, At4g17130

Anwendungsinformationen

Applikationshinweise: Recommended Dilution 1 : 5 000 with standard ECL (WB). Additional Information: Peptide used to elicit this antibody is not conserved in NADPH dependentanzymes, partially conserved across eukaryotic Idh subunits. Some conservationacross bacterial which contain the NAD-dependent form of Idh (as opposed to theNADP-dependent form).

Kommentare:

cellular [compartment marker] of mitochondrial matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-Isocitrate Dehydrogenase Antikörper (ABIN3197487)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Lee, Lee, Yoo, Duncan, Oh, Lee, Lee, Whelan, Hwang: "Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs." in: The Plant cell, Vol. 24, Issue 12, pp. 5037-57, (2013) (PubMed).

Szal, Jastrz?bska, Kulka, Lea: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." in: Planta, Vol. 232, Issue 6, pp. 1371-82, (2010) (PubMed).

Details zu Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Hintergrund: AGI Code: At4g35260
Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.

Molekulargewicht: expected: 39 kDa, apparent: 45 kDa (Arabidopsis thaliana)

UniProt: P93032