TCR gamma/delta Antikörper

Produktnummer ABIN319644

Der Hamster Monoklonal anti-TCR gamma/delta Antikörper (Klon GL3) (ABIN319644) detektiert spezifisch TCR gamma/delta in FACS, IP und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für TCR gamma/delta Antikörper (ABIN319644)

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser TCR gamma/delta Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: GL3

Produktdetails anti-TCR gamma/delta Antikörper

Aufreinigung: Affinity Chromatography

Immunogen: C57BL/6J intra epithelial lymphocytes. Remarks: Spleen cells from immunised Armenian hamster were fused with cells of the murine myeloma SP2/0 myeloma cell line.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1:2 - 1:20, use 10 μL of diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Andere Bezeichnung: T Cell Receptor / TCR gamma/delta

Hintergrund: T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The TCR gamma/delta heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like variable and constant domains. It is expressed during thymopoiesis and on the small subpopulation of gamma/delta TCR expressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are gamma/delta+ and have limited receptor diversity. The TCR gamma/delta heterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant chains leads to T cell activation.Synonyms: T-Cell Receptor delta, T-Cell Receptor gamma, T-Cell Receptor gamma delta, TCRD, TCRG