FLIP Antikörper (C-Term)

Produktnummer ABIN318973

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch FLIP in WB, IF und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für FLIP Antikörper (C-Term) (ABIN318973)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FLIP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-FLIP Antikörper

Bindungsspezifität: AA 191-209, C-Term

Spezifität: This antibody recognises the apoptosis related protein designated FLIP (FLICE inhibitory protein).

Aufreinigung: Purified

Immunogen: A peptide corresponding to aa191 - 209 at the C-terminus of human FLIP delta/FLIPs form.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections: 2g/mL. Western Blot: 1: 1000, detects bands of approximately 35 kDa and 25 kDa in Jurkat celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide (NaN3)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Andere Bezeichnung: CFLAR / Casper / I-FLICE

Hintergrund: FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1. FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. AHP1591 recognizes human and murine delta and gamma variants but not the alpha or beta, variants. FLIP is widely expressed in human tissues and a diverse range of cell lines.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP

Gen-ID: 8837

NCBI Accession: NP_001120655

UniProt: O15519

Pathways: Apoptose, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development