PDCD4 Antikörper (C-Term)

Produktnummer ABIN318876

Dieses Anti-PDCD4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PDCD4 in WB und EIA. Geeignet für Human, Maus, Ratte und Xenopus laevis.

Kurzübersicht für PDCD4 Antikörper (C-Term) (ABIN318876)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDCD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-PDCD4 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects a specific epitope within the carboxy-terminal of programmed cell death 4 (Pdcd4), a novel tumour suppressor.

Aufreinigung: Purified

Immunogen: Synthetic peptide corresponding to amino acids near the carboxy terminal of human Pdcd4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on paraffin sections: 1: 100 - 1: 300, requires protein digestionpretreatment of paraffin sections e. g. trypsin or pronase. ELISA: 1: 10000 - 1: 50000. Western Blot: 1: 1000 - 1: 10000, detects a band of approximately 52 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.01 % Sodium Azide (NaN3)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C

Informationen zur Lagerung: Store at +4 °C or at -20 °C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4

Hintergrund: Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis. Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility. Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells. Pdcd4 expression is down regulated with tumour progression in carcinomas of the lung, colon, prostate and breast.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gen-ID: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6