RAD9A Antikörper (AA 1125-1139)

Produktnummer ABIN318843

Der Kaninchen Polyklonal anti-RAD9A Antikörper (ABIN318843) detektiert spezifisch RAD9A in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Saccharomyces cerevisiae.

Kurzübersicht für RAD9A Antikörper (AA 1125-1139) (ABIN318843)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD9A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-RAD9A Antikörper

Bindungsspezifität: AA 1125-1139

Aufreinigung: Purified

Immunogen: Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 2000 - 1: 4000. Western Blot: 1: 50 - 1: 250, detects a band of approximately 60 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide (NaN3)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Andere Bezeichnung: RAD9A

Hintergrund: In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.Synonyms: Cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC=3.1.11.2, RAD-9A

NCBI Accession: NP_004575

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus