Aurora Kinase B Antikörper (AA 227-238)

Produktnummer ABIN318827

Der Kaninchen Polyklonal Anti-Aurora Kinase B-Antikörper wurde für WB und EIA validiert. Er ist geeignet, Aurora Kinase B in Proben von Human, Maus, Ratte, Affe, Rind (Kuh), Schwein und Hund zu detektieren.

Kurzübersicht für Aurora Kinase B Antikörper (AA 227-238) (ABIN318827)

Target: Aurora Kinase B (AURKB)

Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh), Schwein, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aurora Kinase B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Aurora Kinase B Antikörper

Bindungsspezifität: AA 227-238

Spezifität: This antibody detects Aurora-B kinase (AurB).

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Bovine, Mouse, Chimpanzee, Dog, Pig.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 227-238 of human Aurora-B kinase.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 2000 - 1: 10000. Western Blot: 1: 250 - 1: 1000, detects a band of approximately 39 kDa in HeLa cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Andere Bezeichnung: Aurora Kinase B

Hintergrund: Aurora-B kinase is a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localisation, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis- one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12

Gen-ID: 9212

NCBI Accession: NP_004208

UniProt: Q96GD4

Pathways: T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades