Complement C4A/C4B (AA 1241-1256) Antikörper

Produktnummer ABIN318426

Der Kaninchen Polyklonal anti-Complement C4A/C4B Antikörper (ABIN318426) detektiert spezifisch Complement C4A/C4B in IHC (fro) und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Complement C4A/C4B (AA 1241-1256) Antikörper (ABIN318426)

Target: Complement C4A/C4B

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Produktdetails

Bindungsspezifität: AA 1241-1256

Spezifität: This antibody is specific for complement split product C4d.

Aufreinigung: Purified Ig prepared by affinity chromatography on Protein G

Immunogen: Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections: 1/20 - 1/80. Immunohistochemistry on paraffin sections: 1/20 - 1/80, Heat induced epitope retrieval isrecommended. Immunofluorescence: 1/20 - 1/80.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % Sodium Azide (NaN3)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Complement C4A/C4B

Hintergrund: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.Synonyms: CPAMD2, CPAMD3, Complement Component 4

Gen-ID: 720

NCBI Accession: NP_009224

UniProt: P0C0L4