Cathepsin G Antikörper (Internal Region)

Produktnummer ABIN3183694

Dieses unabhängig validierte Kaninchen Polyklonal-Antikörper erkennt spezifisch Cathepsin G in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Cathepsin G Antikörper (Internal Region) (ABIN3183694)

Target: Cathepsin G (CTSG)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cathepsin G Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Cathepsin G Antikörper

Bindungsspezifität: Internal Region

Spezifität: Cathepsin G Polyclonal Antibody detects endogenous levels of Cathepsin G protein.

Produktmerkmale: Rabbit Polyclonal to Cathepsin G.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Cathepsin G.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, ELISA 1:40000,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (UP)

Validierung #102721 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102721

Datum: 05.11.2018

Antigen: CTSG

Chargennummer: 022204892

Validierte Anwendung: Unfolding Profile

Positivkontrolle: ABIN3183694

Bewertung:

Passed. ABIN3183694 showed T_i at 85.2°C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

Validierungsbilder

p>Unfolding profile of ABIN3183694. The fluorescence signal is plotted against temperature. The native (folded) protein has a high signal at the beginning of the experiment, which decreases and then again increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 85.2°C.

Protokoll

Primärantikörper: ABIN3183694

Full Protocol:

• Dilute ABIN3183694 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Anmerkungen:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C.

Details zu Cathepsin G

Target: Cathepsin G (CTSG)

Andere Bezeichnung: Cathepsin G

Molekulargewicht: 22 kDa

Gen-ID: 1511

UniProt: P08311

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones