PDCD4 Antikörper (C-Term)

Produktnummer ABIN318336

Produktdetails anti-PDCD4 Antikörper

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Xenopus laevis, Staphylococcus aureus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDCD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.

Kreuzreaktivität (Details): Species reactivity (expected):A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from Human, Mouse, Rat and Xenopus based on 100 % homology with the immunizing sequence.
Species reactivity (tested):Human, Mouse, Rat, S. aureus, Xenopus.

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding amino acids near the carboxyl terminus of Human Pdcd4 protein.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/10,000 (a band approximately 52 kDa in size corresponding toPdcd4 protein in the appropriate cell lysate or extract). Immunohistochemistry: 1: 100-1/300.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.06 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4 (PDCD4 Produkte)

Synonyme: H731 antikoerper, D19Ucla1 antikoerper, Ma3 antikoerper, Tis antikoerper, Dug antikoerper, cb45 antikoerper, pdcd4 antikoerper, sb:cb45 antikoerper, wu:fc84b08 antikoerper, h731 antikoerper, MGC69337 antikoerper, PDCD4 antikoerper, wu:fa55g03 antikoerper, wu:fb08a11 antikoerper, wu:fe01a02 antikoerper, zgc:66311 antikoerper, programmed cell death 4 antikoerper, programmed cell death 4b antikoerper, programmed cell death 4 L homeolog antikoerper, programmed cell death 4 (neoplastic transformation inhibitor) antikoerper, programmed cell death 4a antikoerper, PDCD4 antikoerper, Pdcd4 antikoerper, pdcd4b antikoerper, pdcd4.L antikoerper, pdcd4 antikoerper, pdcd4a antikoerper

Hintergrund: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Gen-ID: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6