S1PR1 Antikörper

Produktnummer ABIN317897

Der Kaninchen Polyklonal anti-S1PR1 Antikörper wird verwendet zum Nachweis von S1PR1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IF.

Kurzübersicht für S1PR1 Antikörper (ABIN317897)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S1PR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-S1PR1 Antikörper

Spezifität: EDG-1 antibody detects endogenous levels of EDG-1 protein.(region surrounding Ser232)

Aufreinigung: Affinity chromatography

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Andere Bezeichnung: EDG-1 / S1PR1

Hintergrund: The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. EDG-6, which is expressed in lymphoid and hematopoietic tissues and in lung, signals through Gi/o proteins, which activate growth related pathways.Synonyms: CHEDG1, D1S3362, ECGF1, EDG1, Endothelial differentiation G-protein coupled receptor 1, FLJ58121, S1P receptor 1, S1PR1, Sphingosine 1-phosphate receptor 1

Molekulargewicht: approx. 50 kDa

Gen-ID: 1901

NCBI Accession: NP_001391

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2