S1PR1 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7887142

Der Kaninchen Polyklonal anti-S1PR1 Antikörper wird verwendet zum Nachweis von S1PR1 in Proben von Human. Er wurde validiert für WB, IF, IHC, FACS, LCI und IC.

Kurzübersicht für S1PR1 Antikörper (Extracellular, N-Term) (ABIN7887142)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S1PR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Produktdetails anti-S1PR1 Antikörper

Bindungsspezifität: AA 17-30, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat - 13,14 amino acid residues identical

Produktmerkmale: Anti-S1PR1 (EDG1) (extracellular) Antibody (ABIN7043771, ABIN7045270 and ABIN7045271) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize S1PR1 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 5 mg per reaction

ICC: 1:50

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7237267)

Blocking Peptide: (ABIN7237267)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Andere Bezeichnung: S1PR1

Hintergrund:

Synonyms: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1

Description: Lysophospholipids constitute a group of important lipid mediators, lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG), S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.Sphingosine 1-phosphate receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult5.

Gen-ID: 1901

NCBI Accession: NM_001400

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2