PAK4 Antikörper

Produktnummer ABIN317882

Der Kaninchen Polyklonal anti-PAK4 Antikörper wird verwendet zum Nachweis von PAK4 in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für PAK4 Antikörper (ABIN317882)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PAK4 Antikörper

Spezifität: PAK4/5/6 antibody detects endogenous levels of PAK4/5/6 protein.(region surrounding Val469)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse and Rat.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % Sodium Azide as preservative, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Andere Bezeichnung: PAK4

Hintergrund: Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.Synonyms: KIAA1142, KIAA1264, PAK 4, PAK-4, PAK-5, PAK-5, PAK-6, PAK-7, PAK5, Serine/threonine-protein kinase PAK 4, Serine/threonine-protein kinase PAK 6, Serine/threonine-protein kinase PAK 7, p21-activated kinase 4, p21-activated kinase 5, p21-activated kinase 6, p21-activated kinase 7

Molekulargewicht: approx. 80 kDa

Gen-ID: 10298

NCBI Accession: NP_001014831

UniProt: O96013

Pathways: RTK Signalweg