LIPE Antikörper

Produktnummer ABIN317711

Der Kaninchen Polyklonal anti-LIPE Antikörper wird verwendet zum Nachweis von LIPE in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für LIPE Antikörper (ABIN317711)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIPE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-LIPE Antikörper

Spezifität: This antibody detects endogenous levels of HSL protein.(region surrounding Ala546)

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu LIPE

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Andere Bezeichnung: LIPE

Hintergrund: HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.Synonyms: EC=3.1.1.79, HSL, Hormone-sensitive lipase, LIPE

Molekulargewicht: approx. 115.0 kDa

Gen-ID: 3991

NCBI Accession: NP_005348

UniProt: Q05469

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism