RYR1 Antikörper (C-Term)

Produktnummer ABIN317452

Dieses Maus Monoklonal-Antikörper erkennt spezifisch RYR1 in WB, IF, EIA, IHC (fro) und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund und Schwein.

Kurzübersicht für RYR1 Antikörper (C-Term) (ABIN317452)

Target: RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RYR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Klon: G-1

Produktdetails anti-RYR1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody reacts with the C-terminus cytoplasmic domain of Ryanodine Receptor. Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. This antibody reacts with Ryanodine Receptor (~500 kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).

Aufreinigung: Purified

Immunogen: Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodinereceptor

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections: 1: 100 (Use indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescentmicroscopy will not be adequate to detect the RyR. Additionally, fluorescent, notenzymatic, detection is required. Due to the intensity of confocal lasers, use of ananti-fading agent, such as DABCO, is strongly recommended. ). Immunofluorescence: 1: 100. ELISA: 1: 1000. Flow Cytometry: 1: 500. Western blot: 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu RYR1

Target: RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))

Andere Bezeichnung: Ryanodine Receptor 1 / RYR1

Gen-ID: 6261

UniProt: P21817

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development