RYR1 Antikörper (Intracellular, N-Term)

Produktnummer ABIN7887080

Der Kaninchen Polyklonal anti-RYR1 Antikörper (ABIN7887080) detektiert spezifisch RYR1 in WB, IHC, IF und IC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für RYR1 Antikörper (Intracellular, N-Term) (ABIN7887080)

Target: RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RYR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-RYR1 Antikörper

Bindungsspezifität: AA 1371-1386, Intracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Ryanodine Receptor 1

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse - identical

Produktmerkmale: Anti-Ryanodine Receptor 1 Antibody (ABIN7043604, ABIN7045211 and ABIN7045212) is a highly specific antibody directed against an epitope of human RyR1. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR1 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human RyR1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IP|IHC|IFC

Negative Control: (ABIN7236738)

Blocking Peptide: (ABIN7236738)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu RYR1

Target: RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))

Andere Bezeichnung: RYR1

Hintergrund:

Synonyms: RyR1, Skeletal muscle-type ryanodine receptor

Description: It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1), the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.RyR1 is expressed predominantly in skeletal muscle and in areas of the brain, human RyR1 has at least three known alternative splice variants.Several diseases are attributed to mutations in the RyR1 gene: Central core disease (CCD), multi-minicore disease (MmD) and Malignant hyperthermia (MH) 4.

Gen-ID: 6261

NCBI Accession: NM_000540

UniProt: P21817

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development