HES1 Antikörper (AA 24-35)

Produktnummer ABIN307170

Dieses Anti-HES1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HES1 in WB, IHC, IF, IHC (p) und IHC (fro). Geeignet für Human, Maus, Ratte, Rind (Kuh), Kaninchen, Zebrafisch (Danio rerio), Pferd, Affe, Fledermaus, Xenopus laevis und Hamster. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für HES1 Antikörper (AA 24-35) (ABIN307170)

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Zebrafisch (Danio rerio), Pferd, Affe, Fledermaus, Xenopus laevis, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HES1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-HES1 Antikörper

Bindungsspezifität: AA 24-35

Marke: IHC-plus™

Spezifität: Recognizes human HES-1 (Hairy family).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%) Newt, Smelt (91%) Chicken (82%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa24-35 (TPDKPKTASEH) OF HES1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%), Newt, Smelt (91%), Chicken (82%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1:200 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Human neural stem cells, Raji cell lysate.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Referenzen für anti-HES1 Antikörper (ABIN307170)

Moya, Umans, Maas, Pereira, Beets, Francis, Sents, Robertson, Mummery, Huylebroeck, Zwijsen: "Stalk cell phenotype depends on integration of Notch and Smad1/5 signaling cascades." in: Developmental cell, Vol. 22, Issue 3, pp. 501-14, (2012) (PubMed).

Details zu HES1

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Andere Bezeichnung: HES1 / HES-1

Hintergrund: Name/Gene ID: HES1

Synonyms: HES1, BHLHb39, HHL, HL, Hairy and enhancer of split 1, Hairy homolog, HRY, Hairy homolog (Drosophila), Transcription factor HES-1, Hairy-like protein, HES-1

Gen-ID: 3280

UniProt: Q14469

Pathways: DNA Reparatur