MYOD1 Antikörper

Produktnummer ABIN307158

Der Maus Monoklonal anti-MYOD1 Antikörper (Klon 5-8A) (ABIN307158) detektiert spezifisch MYOD1 in ELISA, IF, IP, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Huhn.

Kurzübersicht für MYOD1 Antikörper (ABIN307158)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 5-8A

Produktdetails anti-MYOD1 Antikörper

Verwendungszweck: Mouse anti Human MyoD1

Kreuzreaktivität: Huhn, Human, Maus, Ratte

Produktmerkmale: Recognizes a protein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilm79s tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing79s sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm79s tumors.

Aufreinigung: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 μg/mg of protein lysate),and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MyoD1

Hintergrund: Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3

Molekulargewicht: 45kDa

UniProt: P15172, P10085, Q02346, P16075

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development