MYOD1 Antikörper

Produktnummer ABIN306848

Der Maus Monoklonal Anti-MYOD1-Antikörper wurde für WB, ELISA, IF, IP, IHC (p) und IHC (fro) validiert. Er ist geeignet, MYOD1 in Proben von Human zu detektieren.

Kurzübersicht für MYOD1 Antikörper (ABIN306848)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 5-2F

Produktdetails anti-MYOD1 Antikörper

Kreuzreaktivität: Huhn, Human, Maus, Schwein, Ratte

Produktmerkmale: Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3,X1414M monoclonal antibody, clone 5.2F recognizes a protein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue.

Aufreinigung: Protein A/G Chromatography

Immunogen: Recombinant mouse MyoD1 protein.,Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/mL) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 μg/mL). These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified monoclonal antibody 5.2F in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MyoD1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development