MITF Antikörper (N-Term)

Produktnummer ABIN306846

Der Maus Monoklonal anti-MITF Antikörper (Klon C5) (ABIN306846) detektiert spezifisch MITF in WB, IHC (p), IP, GS und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für MITF Antikörper (N-Term) (ABIN306846)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MITF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Gel Shift (GS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: C5

Produktdetails anti-MITF Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Mouse anti Human Microphthalmia Transcription Factor (MiTF)

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. MiTF is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteoporotic rat strain harbors a large genomic deletion encompassing the 3 prime half of MiTF including most of the b-HLH-ZIP region. Osteoclasts from these animals lack MiTF protein in contrast to wild-type rat, mouse, and human osteoclasts.

Aufreinigung: Protein A/G Chromatography

Immunogen: N-terminal fragment of human microphthalmia protein.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This antibody can be used for gel supershift assays, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/mL) and immunohistochemistry on frozen and formalin/paraffin tissue sections. Optimal concentration should be evaluated by serial dilutions. In Western blotting, X1405M recognizes a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung: Microphthalmia Transcription Factor (MiTF)

Hintergrund: MiTF

Molekulargewicht: 52kDa,56kDa,70kDa

UniProt: O75030

Pathways: Chromatin Binding