His Tag Antikörper

Produktnummer ABIN306800

Der Maus Monoklonal anti-His Tag Antikörper wird verwendet zum Nachweis von His Tag. Er wurde validiert für WB.

Kurzübersicht für His Tag Antikörper (ABIN306800)

Target: His Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 2C5-1

Produktdetails anti-His Tag Antikörper

Verwendungszweck: Mouse anti His Tag

Sequenz: HHHHHH

Produktmerkmale: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only.

Aufreinigung: Protein A/G Chromatography

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Detects only 6x-His tag by Western blot. Specific for His tags no longer than 6 histidines. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu His Tag

Target: His Tag

Substanzklasse: Tag

Hintergrund: Anti-6x-Histidine Tag Antibody