Phosphotyrosine Antikörper (FITC)

Produktnummer ABIN306700

Der Maus Monoklonal anti-Phosphotyrosine Antikörper wird verwendet zum Nachweis von Phosphotyrosine. Er wurde validiert für WB, IP, IF und EIA.

Kurzübersicht für Phosphotyrosine Antikörper (FITC) (ABIN306700)

Target: Phosphotyrosine

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Klon: PY20

Produktdetails anti-Phosphotyrosine Antikörper (FITC)

Verwendungszweck: Mouse anti Phosphotyrosine, conjugated with FITC

Produktmerkmale: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Aufreinigung: Prot A/G

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: A dilution of 5-10 μg/mL is suggested for immunohistochemistry or immunofluorescence. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Mouse anti phosphotyrosine, PY20, FITC conjugate