ACP1 Antikörper (N-Term)

Produktnummer ABIN306667

Dieser Schaf Polyklonal Antikörper detektiert spezifisch ACP1 in WB und IP. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus und Rind (Kuh).

Kurzübersicht für ACP1 Antikörper (N-Term) (ABIN306667)

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser ACP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-ACP1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Sheep anti Human PTP (HCPTP)

Produktmerkmale: Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18 kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinyl-phosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR. Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1)2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.

Aufreinigung: Ammonium Sulfate Precipitation

Immunogen: The human protein, HCPTPA (GB Accession #M83653) was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Antbibody can be used for Western blotting and immunoprecipitation (1-10 μg/mL. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu ACP1

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Andere Bezeichnung: LMWPTP (HCPTP)

Hintergrund: LmwPTP, sheep anti human low molecular weight protein tyrosine phosphatase, Human Red Cell Protein Tyrosine phosphatase

Molekulargewicht: 18kDa

UniProt: P24666, Q9D358, P41498, P11064