CDKN2D Antikörper

Produktnummer ABIN306573

Der Kaninchen Polyklonal anti-CDKN2D Antikörper (Klon DCS-100) (ABIN306573) detektiert spezifisch CDKN2D in WB, ELISA, IHC (p), IF und RIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CDKN2D Antikörper (ABIN306573)

Target: CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDKN2D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Klon: DCS-100

Produktdetails anti-CDKN2D Antikörper

Kreuzreaktivität: Schwein

Kreuzreaktivität (Details): Pig, cross-reactivity with human < 0.1 % , mouse and rat collagen IV <1 %

Produktmerkmale: hp19, p19 ink4d, CDKN2D, Cyclin-dependent kinase 4 inhibitor D,Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Porcine collagen type IV 100 % , porcine collagens type I, II, and III <0.1 %, porcine elastin, fibronectin and laminin < 0.1 % (RIA at 1:5000 dilution).

Immunogen: Rabbits were immunized with purified collagen type IV from porcine kidney tissue

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,IFA 1:80, IHC(P) 1:500, ELISA >1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Affinity purified antibody lyophilized from phosphate buffered solution, no BSA and preservative added! Reconstitute with 0.1 mL sterile bidistilled water.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu CDKN2D

Target: CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))

Andere Bezeichnung: p19 - ink4d

Pathways: Zellzyklus, Sensory Perception of Sound, Mitotic G1-G1/S Phases, Negative Regulation of intrinsic apoptotic Signaling