BCL2L1 Antikörper (AA 3-14)

Produktnummer ABIN306551

Der Schaf Polyklonal anti-BCL2L1 Antikörper wird verwendet zum Nachweis von BCL2L1 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für BCL2L1 Antikörper (AA 3-14) (ABIN306551)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser BCL2L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-BCL2L1 Antikörper

Bindungsspezifität: AA 3-14

Verwendungszweck: Sheep anti Human bcl-xl

Kreuzreaktivität: Hund, Human, Maus, Ratte

Produktmerkmale: bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs. The larger mRNA gives rise to a protein product, bcl-xl, which is similar in size and predicted structure to bcl-2 (1). The smaller mRNA gives rise to bcl-xS. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2 however membrane bound forms of bcl-x have been demonstrated in thymocytes (2-4). Following the induction of apoptosis all of the bcl-x protein shifts to the membrane form (2). Of the two isoforms of bcl-x, the long (bcl-xl) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression (1,3). Like its homolog bcl-2, bcl-x undergoes phosphorylation, a modification that requires that a specific 60 amino acid loop region be intact, which in turn appears to regulate activity (5,6). Structurally, based on 3D-structure analysis, bcl-x forms pH sensitive cation-selective ion channels in membranes a property shared with the pore forming domains of several bacterial toxins (7). Bcl-xl has been shown to modify the cell79s response to oxidants, to participate in resistance to chemotherapeutic agents and radiation, and to play a key role in the development of the developing CNS (8-10).

Aufreinigung: Ammonium Sulfate Precipitation

Immunogen: A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application: Western Blot at 1-5 μg/mL. Positive control MCF-7 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: bcl-xl

Hintergrund: Hbcl-xl, sheep anti human bcl-xl, bclxl, bcl xl, bclx, bcl2L1, BCL2L

UniProt: Q07817

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling