TJP2 Antikörper (C-Term)

Produktnummer ABIN305886

Dieses Anti-TJP2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TJP2 in WB, IHC, IF und IHC (p). Geeignet für Human, Maus, Ratte und Hund.

Kurzübersicht für TJP2 Antikörper (C-Term) (ABIN305886)

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TJP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TJP2 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Recognizes the ~130-160kD human ZO-2 protein, isoforms A or C. A band of unknown origin lower than 75kD may be observed in certain lysates.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of human or canine ZO-2.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IF (2.5 μg/mL), IHC, IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunofluorescence: 2.5 μg/mL. Cells need to be fixed with ice-cold ethanol for 30 minutes on ice prior to assays. Positive control: Western Blot: CaCO2 human colorectal adenocarcinoma, MDCK II canine kidney, KNRK rat kidney, and TMCK-1 mouse kidney cell lysates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C, Long term: aliquot to Avoid freeze-thaw cycles, and store at -20°C.

Details zu TJP2

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Andere Bezeichnung: TJP2 / ZO2 / ZO-2

Hintergrund: Name/Gene ID: TJP2
Family: Occludin

Synonyms: TJP2, C9DUPq21.11, DUP9q21.11, DFNA51, ZO-2, Zona occludens 2, Zonula occludens protein 2, Tight junction protein 2, Tight junction protein ZO-2, ZO2, X104, Zona occludens protein 2

Gen-ID: 9414

UniProt: Q9UDY2