Thrombospondin 1 Antikörper

Produktnummer ABIN305761

Dieser Maus Monoklonal Antikörper detektiert spezifisch Thrombospondin 1 in WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Thrombospondin 1 Antikörper (ABIN305761)

Target: Thrombospondin 1 (THBS1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Thrombospondin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Thrombospondin 1 Antikörper

Spezifität: Designed for sensitive detection of TSP by Western Blot. It shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Species cross-reactivity: human.

Aufreinigung: Protein G purified

Immunogen: Purified human TSP.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Reduced and non-reduced. 0.5-1.0 μg/mL for 2 hours at RT. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4.

Konservierungsmittel: Azide free

Handhabung: Aliquot to Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Details zu Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Andere Bezeichnung: Thrombospondin 1

Pathways: Autophagie