Cytochrome C Antikörper (AA 1-80)

Produktnummer ABIN305748

Der Maus Monoklonal anti-Cytochrome C Antikörper (Klon 7H8-2C12) (ABIN305748) detektiert spezifisch Cytochrome C in WB, IHC, IHC (p) und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Pferd, Taube, Drosophila melanogaster und Hamster.

Kurzübersicht für Cytochrome C Antikörper (AA 1-80) (ABIN305748)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reaktivität: Human, Ratte, Maus, Pferd, Taube, Drosophila melanogaster, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Klon: 7H8-2C12

Produktdetails anti-Cytochrome C Antikörper

Bindungsspezifität: AA 1-80

Spezifität: An approx. 15 kD band is observed. This recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached).

Aufreinigung: Protein G purified

Immunogen: Synthetic peptide from pigeon CYCS / Cytochrome c.

Type of Immunogen: Synthetic peptide

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Pigeon

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: CYCS / Cytochrome c

Hintergrund: Name/Gene ID: CYCS

Synonyms: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

Gen-ID: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity