ATG12 Antikörper (Internal Region)

Produktnummer ABIN305747

Der Kaninchen Polyklonal anti-ATG12 Antikörper wird verwendet zum Nachweis von ATG12 in Proben von Human und Maus. Er wurde validiert für WB, IHC, ELISA, IP und IHC (p).

Kurzübersicht für ATG12 Antikörper (Internal Region) (ABIN305747)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ATG12 Antikörper

Bindungsspezifität: Internal Region

Spezifität: Recognizes human Apg12 protein at ~22kD, as well as additional bands between 40 and 80kD of unknown origin. Reactivity has been confirmed with Apg12-transfected, overexpressed HEK293 cell lysates.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the internal region of the human Apg12 protein.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 7 μg/reaction.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Andere Bezeichnung: APG12 / ATG12

Hintergrund: Name/Gene ID: ATG12

Synonyms: ATG12, Autophagy-related protein 12, APG12L, Autophagy related 12, FBR93, APG12, APG12-like, Ubiquitin-like protein ATG12, HAPG12

Gen-ID: 9140

UniProt: O94817

Pathways: Autophagie