ErbB2/Her2 Antikörper (AA 1243-1255)
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- Target Alle ErbB2/Her2 Antikörper anzeigen
- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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Bindungsspezifität
- AA 1243-1255
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Reaktivität
- Human, Maus, Ratte, Hund, Affe, Pferd, Schwein, Hamster
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser ErbB2/Her2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Marke
- IHC-plus™
- Spezifität
- Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%) Elephant, Bat, Bovine (92%) Opossum (85%).
- Aufreinigung
- Protein A purified
- Immunogen
- A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%), Elephant, Bat, Bovine (92%), Opossum (85%).
- Isotyp
- IgG
- Top Product
- Discover our top product ErbB2/Her2 Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 μg immunoprecipitated c-erbB-2 from 500 μg of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- 10 mM PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
- Andere Bezeichnung
- ERBB2 / HER2 (ErbB2/Her2 Produkte)
- Synonyme
- CD340 antikoerper, HER-2 antikoerper, HER-2/neu antikoerper, HER2 antikoerper, MLN 19 antikoerper, NEU antikoerper, NGL antikoerper, TKR1 antikoerper, Erbb-2 antikoerper, Neu antikoerper, c-erbB2 antikoerper, c-neu antikoerper, mKIAA3023 antikoerper, wu:fv70f10 antikoerper, zgc:63601 antikoerper, erb2 antikoerper, erb-b2 receptor tyrosine kinase 2 antikoerper, ERBB2 antikoerper, Erbb2 antikoerper, erbb2 antikoerper
- Hintergrund
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Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase
Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1 - Gen-ID
- 2064
- UniProt
- P04626
- Pathways
- RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development
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